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🌱 來自: Huppert’s Notes

Systemic Fungal Infections🚧 施工中

Systemic Fungal Infections

•   Pathogens:

-   See Tables 8.7 and 8.8 for organisms that cause systemic and opportunistic fungal infections

•   Diagnostic tests to evaluate for fungal infections:

-   1,3-Beta-D-glucan (BDG): Tests for a cell-wall polysaccharide that is present in most, but not all, fungi. It is 75–85% sensitive and specific for a fungal infection.

   Relevant fungi for which it tests include:

•   Candida

•   Aspergillus

•   Pneumocystis

•   Histoplasma

•   Coccidioides

   It does not detect mucormycosis (e.g., Rhizopus spp. and Mucor spp.), Cryptococcus spp., and Blastomyces spp.

   False positives can occur with administration of IVIG, albumin, and some hemodialysis filters

-   Galactomannan: Tests for a specific component of the cell wall in Aspergillus. Sensitivity of ~70% in serum. More sensitive on BAL samples (~80%). Rarely, false positives can occur in patients receiving TPN. Piperacillin/tazobactam causes false positives, but less commonly now due to new formulations of the antibiotic

-   Cryptococcal antigen (CrAg): Tests for an antigen associated with Cryptococcus. The serum test is useful in patients with HIV/AIDS and CD4 counts <100 cells/μL.

   If serum CrAg is negative, there is a very low likelihood that the patient has cryptococcal meningitis (especially in HIV, may not be as sensitive in other types of immunocompromise)

   If serum CrAg is positive, get an LP to rule out cryptococcal meningitis in all patients

   If LP demonstrates crypto meningitis, treatment includes induction therapy with amphotericin B + flucytosine, followed by consolidation and maintenance therapy with fluconazole once repeat LP demonstrates clearance of the organism from CSF

-   Histoplasma antigen: Tests for an antigen associated with histoplasmosis. Can be tested off of serum or urine; >90% sensitivity (urine) and 80% sensitivity (serum). Urine Histoplasma antigen is very sensitive for disseminated histoplasmosis in HIV infection.

-   Cocci immunofixation and complement fixation: Used to evaluate for coccidioidomycosis. Different sensitivity/specificity for these tests in blood vs. CSF.

•   Treatment: Antifungal agents – see Table 8.24