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clinical pharmacology of HD-MTX containing regimen

  • MTX enters the cell using the same carrier protein that transports natural folates and leucovorin.
  • Reduced or absent transport capability in some tumors allows MTX to enter the cell through passive diffusion due to high extracellular concentrations.
  • Folate binding proteins, which may be more abundant in malignant cells than in normal cells, can mediate MTX entry through endocytosis.