NOTE

🌱 created from: diagnostic evaluation of chronic myelogenous leukemia (CML)

molecular_diagnostics-of-chronic_myelogenous_leukemia

• BMBx: Advised at dx for CP, if questions of AP/BC (anytime) & loss/failure of cytogenetic response
• Cytogenetics: Testing for BCR-ABLE1 (& to identify any additional chromosomal abnormalities), repeat if significant ↑ qPCR or minimal ↓ (eg, loss of CCyR, CHR)
• FISH: For BCR–ABLE1 fusion gene; historically poorer prog w/ 9q deletion
• Quantitative RT-PCR (qPCR): Fusion mRNA product, most sensitive technique to detect MRD; used extensively to monitor therapy response (early into deep remission)
• Kinase domain mutational analysis: Not obtained at dx (unless BC), only if failure of TKI