Info
automated blood cell counting
- In addition to complete blood counts (CBCs) and the traditional 5-part leukocyte differential:
- neutrophils
- lymphocytes
- monocytes
- eosinophils
- basophils
- NOTE: 基本 5 大血球
newer hematology analyzers can also provide
- quantitative and qualitative information about:
- reticulocytes
- nucleated red blood cells (NRBCs)
- immature granulocytes
- platelet parameters, such as platelet immaturity
- Because of:
- the large number of cells counted
- analysis using multiple physical principles and sophisticated software:
- “Figure 12-1”: most analyzers use at least 2 techniques
- hematology analyzers produce accurate and precise CBCs and leukocyte differential counts, with the exception of basophils because of their low numbers.
- Many laboratories no longer report band neutrophils because:
- accurate and precise identification by automated and morphologic techniques is poor
- their clinical significance (if any) appears minimal, with the possible exception of:
- neonatal sepsis
- febrile children with sickle cell disease:
- to identify bacterial infection in the setting of vaso-occlusive crisis
- In most clinical circumstances:
- total white blood cell (WBC) count
- automated absolute neutrophil count:
- are better tests for identifying infection
- Hematology analyzers provide excellent sensitivity to distinguish between normal and abnormal samples via operator alerts (flags) prompting:
- microscopic review of a stained peripheral blood film for selected samples